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KMID : 0359319960360010075
Korean Journal of Veterinary Research
1996 Volume.36 No. 1 p.75 ~ p.82
The effect of Westergren tube angle , temperature and osmobility with the different percent of NaCl solution on erythrocyte sedimentation rate



Abstract
Erythrocyte sedimentation rate is influenced by plasma protein, red cell itself and physiological conditions and it is clear that the measurement of erythrocyte sedimentation rate varies with technique and various environmental factors.
The effect of temperature, angle of test tube, and osmobility with different percent of NaCl solution on erythrocyte sedimentation rate on sheep was determined by modified Westergren method.
In sheep, as the angle of Westergren tube was decreased from 90¡Æ to 45¡Æ, erythrocyte sedimentation rate was increased in both diluted plasma and NaCl solutions.
As temperature was increased from 4¡É to 20¡É at 45¡É¡Æ angle. erythrocyte sedimentation rate was increased.
In ruminants, this modified Westergren methods, erythrocyte 1: plasma 9 instead of erythrocyte 4: plasma 6(whole blood), enable them to have meanings as dog therefor, using this method, clinical can determine the erythrocyte sedimentation rate of ruminants for diagnosis.
Because erythrocyte sedimentation rate was changed according to the angle of Westergren tube, temperature as erythro cyte diluted with NaCl, this study detected that the change of temperature, the angle of Westergren tube could change erythrocyte sedimentation rate by effecting red cell itself.
The increase of osmobility owing to change of NaCl percent resulted in the decrease of rapid erythrocyte sedimentation rate. So this fact indicate that deformibility and the change of red cell volume have meaning in the change of erythrocyte sedimentation rate.
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